A8097
Acridine Orange hydrochloride solution
10 mg/mL in H2O, >95.0% (HPLC)
Synonym(s):
3,6-Bis(dimethylamino)acridine hydrochloride
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About This Item
Empirical Formula (Hill Notation):
C17H19N3 · HCl
CAS Number:
Molecular Weight:
301.81
Beilstein:
3575587
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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Quality Level
Assay
>95.0% (HPLC)
form
solution
concentration
10 mg/mL in H2O
color
orange to red
εmax
47-54 at 486-492 nm in water
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Cl[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
InChI
1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H
InChI key
VSTHNGLPHBTRMB-UHFFFAOYSA-N
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Application
Acridine Orange hydrochloride solution has been used to study autophagic cell death. It has also been used for the staining of chromosomes.
Biochem/physiol Actions
Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentration it intercalates into DNA and precipitates RNA. However, at high concentration it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It goes into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in the acidic organelle giving a bright red fluorescence.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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