68022

Y. enterocolitica Millichrome^™ plus Supplement

suitable for microbiology

• Synonym(s): Y. enterocolitica chromogenic supplement
• UNSPSC Code: 41171614

Properties

• sterility: sterile; 0.45 μm filtered
• Quality Level: 200
• form: dry powder
• shelf life: limited shelf life, expiry date on the label
• technique(s): microbiological culture: suitable
• solubility: sterile water: 100 mg/mL
• application(s): food and beverages; microbiology
• shipped in: ambient
• storage temp.: 2-8°C
• suitability: selective for bacteria: Yersinia enterocolitica

Description

General description

Y. enterocolitica Millichrome^™ plus Supplement is a selective media supplement for the detection of Yersinia enterocolitica. The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon enzymatic activity of Yersinia enterocolitica.Millichrome^™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 5 vials, with each vial sufficient for 1 liter of final media. The prepared supplement is added to 1 liter of melted Y. enterocolitica Millichrome^™ plus Agar base cooled at 45-50 °C +/- 2 °C.

Application

Y. enterocolitica Millichrome^™ plus Supplement is a selective chromogenic media supplement intended for use in the qualitative detection, differentiation, and presumptive identification of Yersinia enterocolitica from various samples.

Components

Composition per vial:
(1 vial is sufficient for 5 L of final media)
Selective mix 0.5g

Preparation Note

• Prepare a stock solution of the supplement: Add 100 mg to 1 mL of purified water.
• Swirl well until complete dissolution. Filter sterilize at 0.45 μm.
• Warning 2: This supplement stock solution should be used immediately after Preparation, or can be stored at -20 °C and used within 15 days.
• Add 1 mL of the prepared supplement solution to 1 L of the prepared Y. enterocolitica Millichrome^™ plus Agar base cooled at 45-50 °C +/- 2 °C.
• Swirl gently to homogenize.
• Pour into sterile Petri dishes.

Analysis Note

The Pathogenic Y. enterocolitica is detected by a characteristic mauve color after only 36-48 h of incubation, while the non-pathogenic Y. enterocolitica and background flora (Citrobacter, Enterobacter, Aeromonas, etc.) form metallic blue colonies or limited growth in metallic blue color after incubation at the same temperature

Legal Information

MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3