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10814270001

Roche

Primer for cDNA Synthesis

lyophilized, pkg of 40 μg

Synonym(s):

oligo-p(dt)15

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About This Item

UNSPSC Code:
41106300

form

lyophilized

Quality Level

100

packaging

pkg of 40 μg

manufacturer/tradename

Roche

storage temp.

−20°C (−15°C to −25°C)

General description

This primer consists of a 15-mer oligonucleotide that shows thymidine (T) at each individual position. The oligonucleotides are HPLC purified, desalted, and supplied as a lyophilizate of the Li-salt. The p(dT)15 primer is not phosphorylated at the 5′ end.Oligo(dT)N generates full-length cDNAs. If template contains oligo(A) stretches internally, the primer may bind these, causing mis priming and transcripts that are not full-length.

Composition: p(dT)15[ 15-mer oligonucleotide containing only deoxythymidine (dT) residues].

Phosphorylation: The p(dT)15 primer is not phosphorylated at the 5′ end.

Application

Primer for cDNA synthesis may be used in:
  • RT-PCR
  • Generation of cDNA libraries
  • Synthesis of first-strand cDNA

Unit Definition

8 nmol ξ 1 A260 unit

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

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