Skip to Content
Merck
All Photos(4)

Key Documents

View All Documentation

PICM01250

Millipore

Millicell® Standing Cell Culture Inserts

pore size 0.4 μm, diam. 12 mm, transparent PTFE membrane, hydrophilic, size 24 wells, sterile

Synonym(s):

Millicell Cell Culture Insert, 12 mm, hydrophilic PTFE, 0.4 µm, Millicell-CM, cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22

material

polystyrene housing
transparent PTFE membrane

Quality Level

400

sterility

ethylene oxide treated
sterile

feature

hydrophilic

packaging

pack of 50

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

H

10.5 mm

diam.

12 mm

filtration area

0.6 cm2

size

24 wells

surface area

0.6 cm2

working volume

0.6 mL

color

transparent, when wetted

matrix

Biopore™

pore size

0.4 μm

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

General description

Device Configuration: 24-well plate, 0.4μm pore size

With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Millicell® Standing Inserts:
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies

Membrane Type:
Biopore Membrane (hydrophilic PTFE)
- For low protein-binding, live cell viewing, and immunofluorescent applications

Applications
Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry

Application

Research Category
Cell Culture

Packaging

Individually blister packaged

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ming Zeng et al.
Gene, 753, 144810-144810 (2020-05-30)
Meiotic entry and progression require dynamic regulation of germline gene expression. m6A on mRNAs and recognition by YTHDC2 has been known as post-transcriptional regulatory complex, but the roles of this regulator remain unclear for meiotic initiation and progression in female
Andrea S Cupp et al.
Biology of reproduction, 68(6), 2033-2037 (2003-02-28)
The first morphological event after initiation of male sex determination is seminiferous cord formation in the embryonic testis. Cord formation requires migration of pre-peritubular myoid cells from the adjacent mesonephros. The embryonic Sertoli cells are the first testicular cells to
P Hardman et al.
Development, growth & differentiation, 35(6), 683-690 (1993-01-01)
Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane
R Le Bouffant et al.
Human reproduction (Oxford, England), 25(10), 2579-2590 (2010-07-31)
The initiation of meiosis is crucial to fertility. While extensive studies in rodents have enhanced our understanding of this process, studies in human fetal ovary are lacking. We used RT-PCR and immunohistochemistry to investigate expression of meiotic factors in human
Gravity in mammalian organ development: differentiation of cultured lung and pancreas rudiments during spaceflight.
Spooner, B S, et al.
The Journal of Experimental Zoology, 269, 212-222 (1994)
View All Related Papers

Protocols

Toluidine Blue Staining Protocol for Millicell® Culture Inserts

This is a Toluidine Blue Staining protocol listing materials and methods.

Microscopic Examination of Samples

This protocol covers three methods for the microscopic examination of cell samples.

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This technical article covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Related Content

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service