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MAB1920

Sigma-Aldrich

Anti-Laminin γ1 Antibody, clone 2E8

ascites fluid, clone 2E8, Chemicon®

Synonym(s):

Laminin B2 Chain

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2E8, monoclonal

species reactivity

rat, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
cell culture | mammalian: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_002293.3

UniProt accession no.

P11047

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... LAMC1(3915)

Specificity

Reacts with the B2 chain of intact human laminin and does not block the neurite stimulating activity of laminin. Cross reacts with the 200 kDa chain of rat laminin.

Application

ELISA (50% maximal binding to human laminin): (1:300,000.

Immunofluorescence

Affinity chromatography

Immunoprecipitation

Immunoblotting

Optimal working dilutions must be determined by end user.
This Anti-Laminin γ1 Antibody, clone 2E8 is validated for use in ELISA, CULT, IH, IP, WB for the detection of Laminin γ1.

Target description

200 kDa

Physical form

Liquid, no preservatives added.

Analysis Note

Control
Basement membrane, Hippocampal tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jonathan C R Jones et al.
Journal of cell science, 118(Pt 12), 2557-2566 (2005-06-02)
Mechanical ventilation is a valuable treatment regimen for respiratory failure. However, mechanical ventilation (especially with high tidal volumes) is implicated in the initiation and/or exacerbation of lung injury. Hence, it is important to understand how the cells that line the
Laminin alpha 2 (merosin)-deficient muscular dystrophy and demyelinating neuropathy in two cats.
O'Brien, D P, et al.
Journal of the Neurological Sciences, 189, 37-43 (2001)
CARDIOVASCULAR RISK FACTORS AFFECT HIPPOCAMPAL MICROVASCULATURE IN EARLY AD.
Schwartz, E; Wicinski, B; Schmeidler, J; Haroutunian, V; Hof, PR
Translational neuroscience null
Mapping gene expression changes in the fetal rat testis following acute dibutyl phthalate exposure defines a complex temporal cascade of responding cell types.
Johnson, KJ; Hensley, JB; Kelso, MD; Wallace, DG; Gaido, KW
Biology of Reproduction null
Hiroyuki Ido et al.
The Journal of biological chemistry, 279(12), 10946-10954 (2004-01-01)
The adhesive interactions of cells with laminins are mediated by integrins and non-integrin-type receptors such as alpha-dystroglycan and syndecans. Laminins bind to these receptors at the C-terminal globular domain of their alpha chains, but the regions recognized by these receptors
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