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MAB13170

Sigma-Aldrich

Anti-Cav1.2 calcium channel Antibody, clone L57/46

clone L57/46, from mouse

Synonym(s):

calcium channel, voltage-dependent, L type, alpha 1C subunit1, voltage-gated calcium channel alpha subunit Cav1.2, calcium channel, L type, alpha 1 polypeptide, isoform 1, cardic muscle, calcium channel, cardic dihydropyridine-sensitive, alpha-1 subunit,

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

L57/46, monoclonal

species reactivity

mouse, human

species reactivity (predicted by homology)

rabbit (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_001129994

UniProt accession no.

P15381

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CACNA1C(775)
rabbit ... Cacna1C(100101555)

General description

This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Many alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Specificity

This antibody recognizes Cav1.2 calcium channel at the cytoplasmic domain.

Immunogen

Epitope: Cytoplasmic domain
Recombinant protein corresponding to rabbit Cav1.2 calcium channel at the cytoplasmic domain proximal to the C-terminus.

Application

Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Cav1.2 calium channel in human kidney tissue.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
This Anti-Cav1.2 calcium channel Antibody, clone L57/46 is validated for use in WB, IH for the detection of Cav1.2 calcium channel.

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected Cav1.2 calcium channel on 10 µg of mouse brain tissue lysate.

Target description

Observed at ~ 240 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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