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524739

Sigma-Aldrich

Phosphodiesterase 10A2, His•Tag®, Human, Recombinant, S. frugiperda

Synonym(s):

PDE10A2

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About This Item

Enzyme Commission number:
3.1.4.17 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.54

recombinant

expressed in baculovirus infected Sf21 cells

Quality Level

100

form

liquid

specific activity

≥0.25 U/μL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

Full-length, recombinant, human, phosphodiesterdase 10A2 (accession no. 10A2) fused at the N-terminus to a His•Tag7reg; sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation of catalytically active enzyme.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme required to convert 1 µmol cAMP to AMP per min at 30°C, pH 7.5.

Physical form

In RIPA buffer (150 mM NaCl, 10 mM Tris-HCl, 1% NP-40), pH 8.3.

Other Notes

Kotera, J., et. al. 2004. J. Biol. Chem.279, 4366.
Fujishige K., et. al. 1999. Eur. J. Biochem.266, 1118.
Kotera, J., et. al. 1999. Biochem. Biophys. Res. Commun.261, 551.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

EU REACH Annex XIV (Authorisation List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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