516620

Peroxynitrite

Synthesized from isoamyl nitrite and hydrogen peroxide.

• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• color: yellow
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Note: 1 set = 2 x 1 ml.

Synthesized from isoamyl nitrite and hydrogen peroxide. Peroxynitrite (ONOO–) is a strong biological oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine. It has been implicated in a variety of patho-physiological conditions including acute respiratory distress syndrome (ARDS), arthritis, atherosclerosis, inflammatory bowel disease, ischemia-reperfusion, N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-mediated neurotoxicity, and septic shock. This product decomposes or isomerizes at pH 8 or less; the half-life at pH 7.5 is ~1.5 s. ε^{302 nm} = 1670^M-1cm^-1 in 0.1 MNaOH.

Synthesized from isoamyl nitrite and hydrogen peroxide. Peroxynitrite (ONOO^–) is a cell-permeable strong biological oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine. Has been implicated in a variety of pathophysiological conditions including acute respiratory distress syndrome (ARDS), arthritis, atherosclerosis, inflammatory bowel disease, ischemia-reperfusion, N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-mediated neurotoxicity, and septic shock.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Oxidizer (K)

Physical form

Supplied in 4.7% NaOH at 160-200 mM.

Preparation Note

Peroxynitrite is soluble in aqueous buffers that have been pre-purged with an inert gas and pre-cooled to 4°C.

Reconstitution

Protect from heat, light, metal ions, and air. Peroxynitrite is unstable; thaw rapidly, dispense into aliquots, purge tubes with an inert gas, and freeze -70°C. Activity decreases ~2% /day at -20°C.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Marla, S.S., et al. 1997. Proc. Natl. Acad. Sci. USA94, 14243.
Sakuma, S., et al. 1997. Biochem. Biophys. Res. Commun. 230, 476.
Lymar, S.V., et al. 1996. Biochemistry 35, 7855.
Uppu, R.M., and Pryor, W.A. 1996. Methods Enzymol. 269, 322.
Ischiropoulos, H., and al-Medhi, A.B. 1995. FEBS Lett. 364, 279.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS05,GHS08
• Signal Word: Danger
• Hazard Statements: H290 - H314 - H341 - H351
• Precautionary Statements: P202 - P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Carc. 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B
• Storage Class Code: 5.1B - Oxidizing hazardous materials
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable