208900

Calpastatin, Human, Recombinant, Domain I

Domain I of human calpastatin.

• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Quality Level: 100
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: aqueous buffer: 1 mg/mL; water: 1 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

An endogenous protease inhibitor that acts specifically on Calpain I (Cat. No. 208712), a Ca²⁺-dependent cysteine protease. Widely distributed in mammalian and avian cells. Has better inhibitory action than Calpain Inhibitor I (Cat. No. 208719) and Calpain Inhibitor II (Cat. No. 208721). Calpastatin corresponds to domain I of human calpastatin. The inhibitory sequence of human domain I consists of at least 18 amino acid residues.

Domain I of human calpastatin. Endogenous protease inhibitor that acts specifically on Calpain-1 (Cat. No. 208712). Exhibits greater inhibitory potency than calpain inhibitors I and II.

Biochem/physiol Actions

50 nM completely inhibits the activity of 7.5 µg/ml calpain I

Cell permeable: no

Primary Target
calpain-1

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Analysis Note

homogeneous by SDS-PAGE

Other Notes

Salamino, F., et al. 1994. Biochem. Biophys. Res. Commun. 199, 1326.
Uemori, T., et al. 1990. Biochem. Biophys. Res. Commun. 166, 1485.
Asada, K., et al. 1989. J. Enzyme Inhib.3, 49.

Legal Information

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable