1.50479
SeQuant® ZIC®-HILIC 200Å (3.5 µm) HPLC Columns
L × I.D. 150 mm x 0.3 mm capillay Column
Synonym(s):
Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
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About This Item
UNSPSC Code:
41115709
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product name
SeQuant® ZIC-HILIC™ (3.5µm,200Å) 150 x 0.3 mm, suitable for HPLC
material
stainless steel column
Quality Level
Agency
suitable for USP L114
product line
SeQuant®
feature
endcapped: no
parameter
400 bar pressure
70 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable (Capillary LC)
mass spectrometry (MS): suitable (High)
column L × I.D.
15 cm × 0.3 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC bonding phase
particle size
3.5 μm
pore size
200 Å pore size
operating pH
2-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
General description
SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide
Application
- Click postsynthesis of microporous organic network@silica composites: This study presents the creation of novel composites for mixed-mode chromatography, showcasing an innovative approach to chemical separation technologies, which includes hydrophilic interaction liquid chromatography (HILIC) (Sun HF et al., 2023).
- Evaluation of Hold-Up Volume Determination Methods: This research evaluates different methods and markers in HILIC, critical for refining analytical methods in complex sample matrices such as pharmaceuticals, enhancing accuracy and reproducibility in chemical analysis (Redon L et al., 2023).
- Determination of polyphenolic content in berry juices: Utilizing focusing-modulated comprehensive two-dimensional liquid chromatography, this study highlights the application of HILIC in food analysis, demonstrating its effectiveness in extracting detailed nutritional information (Arena K et al., 2023).
- Simple and sensitive determination of sulfites in Chinese herbal teas: This paper employs ultra-high-performance liquid chromatography coupled with mass spectrometry, showing HILIC′s role in ensuring consumer safety by detecting potentially harmful additives in herbal products (Yang L et al., 2022).
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
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