05-740

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12

clone 10H11.E12, Upstate^®, from mouse

• Synonym(s): A-T, mutated, AT mutated, TEL1, telomere maintenance 1, homolog, ataxia telangiectasia mutated, ataxia telangiectasia mutated (includes complementation groups A, C and D), ataxia telangiectasia mutated protein, human phosphatidylinositol 3-kinase homolog
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 10H11.E12, monoclonal
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000042
• UniProt accession no.: Q13315
• shipped in: ambient
• target post-translational modification: phosphorylation (pSer1981)
• Gene Information: human ... ATM(472)

Description

General description

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. The essential requirement for the substrates of ATM/ATR is S/TQ. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S/TQ are negative determinants for substrate phosphorylation. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.

Specificity

Predicted to cross-react with rat based on sequence homology

This antibody recognizes ATM, Mr ~370 kDa. A non-specific protein was also detected, Mr ~ >400 kDa.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 1974-1988 (SLAFEEG[pS]QSTTISS) of human ATM. The immunizing sequence has 11/12 identical amino acids in mouse and rat.

Application

Immunoprecipitation:
Phosphorylated ATM was immunoprecipitated from irradiated HeLa cells (Figure A, lanes 3 and 4).

Immunocytochemistry:
Foci are detected in irradiated human and mouse fibroblasts. Determined by an independent laboratory.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 (Mouse Monoclonal Antibody) validated in ICC, IF, IP, WB to detect phospho-ATM (Ser1981) also known as A-T mutated.

Quality

Routinely evaluated by immunoblot on in crude lysates from irradiated HeLa cells.

Western Blot Analysis:
0.5 µg/mL of this lot detected phosphorylated ATM in crude lysates from irradiated HeLa cells.

Target description

~370 kDa

Physical form

Format: Purified

Protein G Purified

Protein G purified mouse IgG in 0.014 M phosphate buffer, pH 7.6, with 0.175 M NaCl, 0.07 % Sodium Azide and 30% glycerol. Liquid at -20°C.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Irradiated HeLa cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1