Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS

Product Number

Brand

Product Description

267058

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.99% trace metals basis

267007

Sigma-Aldrich

Nickel, foil, thickness 0.25 mm, 99.995% trace metals basis

268259

Sigma-Aldrich

Nickel, foil, thickness 0.125 mm, ≥99.9%

268283

Sigma-Aldrich

Nickel, powder, <1 μm, 99.8% trace metals basis

221678

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 2800, slurry, in H₂O, active catalyst

203904

Sigma-Aldrich

Nickel, powder, <150 μm, 99.99% trace metals basis

510041

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 4200, slurry, in H₂O, active catalyst

510068

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 3202, slurry, in H₂O, active catalyst

510033

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 2400, slurry, in H₂O, active catalyst

357634

Sigma-Aldrich

Nickel, wire, diam. 0.25 mm, ≥99.9%

GF79581864

Nickel, foil, 1m coil, thickness 0.125mm, coil width 50mm, annealed, 99%

GF50091320

Nickel, tube, 1000mm, outside diameter 2.0mm, inside diameter 1.6mm, wall thickness 0.2mm, hard, 99.5%

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GF20717591

Nickel, tube, 200mm, outside diameter 1.0mm, inside diameter 0.8mm, wall thickness 0.1mm, hard, 99.5%

GF38593839

Nickel, foil, 15mm disks, thickness 0.125mm, annealed, 99.98%

GF77657888

Nickel, mesh, 120x120mm, nominal aperture 0.43mm, thickness 0.004mm, wire diameter 0.067mm, 51 wires/inch, open area 75%, electro-formed mesh, 99.9%

GF72359017

Nickel, foil, 50x50mm, thickness 0.25mm, as rolled, 99.999%

GF16048874

Nickel, microfoil, disks, 10mm, thinness 0.25μm, specific density 222.55μg/cm², permanent mylar 3.5μm support, 99.99+%

GF27854196

Nickel, foil, 50x50mm, thickness 1.6mm, 99%

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Nickel, foil, 150x150mm, thickness 0.25mm, annealed, 99%

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Nickel, wire reel, 100m, diameter 0.4mm, as drawn, 99%

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Nickel, foil, 4mm disks, thickness 0.0125mm, 99.9%

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Nickel, foil, 25x25mm, thickness 0.3mm, as rolled, 99.999%

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Nickel, wire reel, 1m, diameter 1.0mm, as drawn, 99.99+%

GF63370100

Nickel, tube, 500mm, outside diameter 1.0mm, inside diameter 0.2mm, wall thickness 0.4mm, hard, 99.5%

GF02040837

Nickel, wire reel, 100m, diameter 0.15mm, as drawn, 99%

GF13415903

Nickel, foil, 4mm disks, thickness 0.0075mm, 99.95%

GF04087076

Nickel, wire reel, 10m, diameter 0.25mm, as drawn, 99%

GF86231729

Nickel, foil, 4mm disks, thickness 0.05mm, as rolled, 99.99+%