G005959
HPLC Analysis of Glycan and Isomers on Proteomix® SAX-NP5, High Resolution Separation
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 μm particles (Z777228)
mobile phase: (A) 2.5% (v/v) acetic acid, 0.5% TEA in water; 0.5% acetic acid in acetonitrile
gradient: 0-100% B in 60 min
flow rate: 0.3 mL/min
detector: Fluorescence, Ex/Em=360/425 nm
sample: 2-AA (anthranilic acid) labeled N-linked oligosaccharide profiling of an IgG1 sample
suitability
application for HPLC
application(s)
life science and biopharma
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