Skip to Content
Merck
All Photos(2)

Key Documents

More Documents

RAB0145

Sigma-Aldrich

Human Dkk-3 ELISA Kit

for serum, plasma, cell culture supernatants and urine

Synonym(s):

Dickkopf-related protein 3, Dkk-3

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 2.74-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... DKK3(27122)

General description

The Human Dickkopf homolog 3 (DKK3) enzyme-linked immunosorbent assay (ELISA) kit is an in vitro enzyme-linked immunosorbent assay used for the quantitative measurement of human DKK-3 in serum, plasma, cell culture supernatants, and urine. Dickkopf homolog 3 (DKK3) protein belongs to the Dkk family. The DKK3 gene acts as a tumor suppressor gene and is suppressed in several human cancers. This gene is mapped on the human chromosome at 11p15.3.

Immunogen

Recombinant Human DKK3

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Dkk-3 ELISA Kit has been used to evaluate Dickkopf homolog 3 (DKK3) from conditioned media. It has also been used to determine DKK3 levels in urine samples.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Francesca Valdora et al.
International journal of molecular sciences, 14(4), 7492-7505 (2013-04-10)
Medulloblastoma (MB) is a malignant pediatric brain tumor arising in the cerebellum consisting of four distinct subgroups: WNT, SHH, Group 3 and Group 4, which exhibit different molecular phenotypes. We studied the expression of Dickkopf (DKK) 1-4 family genes, inhibitors
Adam A Kraya et al.
Autophagy, 11(1), 60-74 (2014-12-09)
Macroautophagy, a catabolic process of cellular self-digestion, is an important tumor cell survival mechanism and a potential target in antineoplastic therapies. Recent discoveries have implicated autophagy in the cellular secretory process, but potential roles of autophagy-mediated secretion in modifying the

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service