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MTOXRTSUP

Sigma-Aldrich

RPTEC Tox Supplement

sterile-filtered, suitable for cell culture, add to 500 ML of basal medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.81

Quality Level

300

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

General description

Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 6 mL of RPTEC Tox media supplement which is added to basal media and other components listed below to produce 500 mL of media needed for optimal culture conditions of Sigma′s Human Renal Proximal Tubule Epithelial Cells (RPTEC) when used for Kidney Toxicology studies. The final RPTEC Tox media was developed specifically for use with Sigma′s human RPTEC control SA7K Clone cells and various knockouts (KO).

Preparation Note

Prepare Tox Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-glutamine (G7513), 6 mL RPTEC Tox Supplement (MTOXRTSUP) and 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Tox Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO2 in air atmosphere.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marianne K Vormann et al.
The AAPS journal, 20(5), 90-90 (2018-08-16)
Proximal tubules in the kidney play a crucial role in reabsorbing and eliminating substrates from the body into the urine, leading to high local concentrations of xenobiotics. This makes the proximal tubule a major target for drug toxicity that needs

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