MAK451
Glucose-6-Phosphate Dehydrogenase Assay Kit
Sufficient for 100 colorimetric tests
Synonym(s):
G6PD Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Quality Level
input
plasma
serum
tissue
cell culture medium
detection method
colorimetric
relevant disease(s)
hematological disorder, anemia
storage temp.
−20°C
General description
Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia.
Application
The Glucose-6-Phosphate Dehydrogenase Assay Kit may be used for:
- Metabolism Research
- Drug Discovery
Features and Benefits
- Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose-6-phosphate dehydrogenase levels across a wide range from 0.2 to 100 U/L, offering both convenience and sensitivity.
- Compatibility with High-Throughput Systems: Effortlessly incorporate our kit into high-throughput handling systems, guaranteeing seamless integration and maintaining efficiency and accuracy in your laboratory operations.
Suitability
The kit is suitable for the quantitative determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism in plasma, serum, tissue and culture media, etc.
Principle
The non-radioactive, colorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit is based on the reduction of tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.
Other Notes
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Muta. 2
Storage Class Code
12 - Non Combustible Liquids
Certificates of Analysis (COA)
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