Skip to Content
Merck
All Photos(2)

Key Documents

More Documents

MAK197

Sigma-Aldrich

Succinate Dehydrogenase Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

SDH Activity Assay Kit

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

neurological disorders

storage temp.

−20°C

Gene Information

human ... SDHA(6389) , SDHAF1(644096) , SDHAF2(54949) , SDHB(6390) , SDHC(6391) , SDHD(6392)
mouse ... SDHA(66945) , SDHAF1(68332) , SDHAF2(66072) , SDHB(67680) , SDHC(66052) , SDHD(66925)
rat ... SDHA(157074) , SDHAF2(361726) , SDHB(298596) , SDHC(289217) , SDHD(363061)

General description

Succinate dehydrogenase (SDH; EC 1.3.5.1) is a mitochondrial enzyme that catalyzes the oxidation of succinate to fumarate and carries electrons from FADH to CoQ in eukaryotes and bacteria. It has a central function in the maintenance of cellular energy metabolism via the Krebs (tricarboxylic acid) cycle and the electron transport chain. Mutations in SDH cause hereditary paraganglioma/phaeochromocytoma syndrome and a neurodegenerative disorder known as Leigh syndrome.

Application

Succinate Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the enzyme activity of succinate dehydrogenase in samples.

Suitability

Suitable for the detection of Succinate Dehydrogenase Activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principle

The Succinate Dehydrogenase Activity Colorimetric Assay kit provides a simple and sensitive procedure for measuring SDH activity in a variety of tissues, cells, and isolated mitochondria. SDH activity is determined by generating a product with absorbance at 600 nm proportional to the enzymatic activity present. One unit of SDH is the amount of enzyme that generates 1.0 μmole of DCIP per minute at pH 7.2 at 25 °C.

Kit Components Only

Product No.
Description
  • SDH Assay Buffer
  • SDH Substrate Mix
  • SDH Probe
  • DCIP Standard, 2 mM
  • SDH Positive Control

replaced by

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Microbial Siderophore Enterobactin Promotes Mitochondrial Iron Uptake and Development of the Host via Interaction with ATP Synthase.
Qi B and Han M
Cell (2018)
Fabrizio Pin et al.
Journal of cachexia, sarcopenia and muscle, 9(4), 685-700 (2018-07-17)
Cachexia frequently occurs in women with advanced ovarian cancer (OC), along with enhanced inflammation. Despite being responsible for one third of all cancer deaths, cachexia is generally under-studied in OC due to a limited number of pre-clinical animal models. We
Growth of ovarian cancer xenografts causes loss of muscle and bone mass: a new model for the study of cancer cachexia.
Pin F, et al.
Journal of Cachexia, Sarcopenia and Muscle, 9(4), 685-700 (2018)
Mohammad Golam Sabbir et al.
Cell communication and signaling : CCS, 19(1), 98-98 (2021-09-27)
The calcium (Ca2+)/calmodulin (CAM)-activated kinase kinase 2 (CAMKK2)-signaling regulates several physiological processes, for example, glucose metabolism and energy homeostasis, underlying the pathogenesis of metabolic diseases. CAMKK2 exerts its biological function through several downstream kinases, therefore, it is expected that depending
Brian P Daniels et al.
Immunity, 50(1), 64-76 (2019-01-13)
As long-lived post-mitotic cells, neurons employ unique strategies to resist pathogen infection while preserving cellular function. Here, using a murine model of Zika virus (ZIKV) infection, we identified an innate immune pathway that restricts ZIKV replication in neurons and is
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service