M0535
Mycoplasma Broth
powder, suitable for microbiology
Synonym(s):
Mycoplasma media
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About This Item
UNSPSC Code:
41106200
NACRES:
NA.74
form
powder
technique(s)
microbiological culture: suitable
solubility
H2O: 25.5 g/L (Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.)
application(s)
microbiology
General description
Mycoplasma Broth is a basal medium which, after enrichment with yeast extract, horse serum, and antibiotics, will support the growth of Mycoplasma sp. The components contained in this basal medium do not exhibit inhibitory or toxic effects when used to culture mycoplasma. A special mineral supplement has been added to improve the growth and colony characteristics of the organism.
Application
Mycoplasma Broth has been used for isolation and cultivation of mycoplasma. It may also be used to grow Porphyromonas gingivalis under anerobic conditions.
Components
Powder contains (g/L): 10.0 bacterial peptone, 10.0 LAB-LEMCO powder, 5.0 sodium chloride, 0.5 mineral supplement.
Preparation Note
Basal Medium:
1. Suspend 25.5 g of powdered medium in 1L of distilled water. Stir until the powder dissolves.
2. Adjust the pH of the solution to 7.8 ± 0.2.
3. Autoclave at 121 °C and 15 psi for 15 minutes to sterilize. Cool to 50 °C and add sterile supplements.
1. Suspend 25.5 g of powdered medium in 1L of distilled water. Stir until the powder dissolves.
2. Adjust the pH of the solution to 7.8 ± 0.2.
3. Autoclave at 121 °C and 15 psi for 15 minutes to sterilize. Cool to 50 °C and add sterile supplements.
Storage and Stability
Store dry powdered medium at room temperature and prepare medium at 2-8 °C.
Other Notes
The product is intended for laboratory use only.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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