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Merck
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Key Documents

L6715

Sigma-Aldrich

Biocoll

liquid, sterile-filtered, BioReagent, suitable for hybridoma

Synonym(s):

Separation Medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered
sterile

Quality Level

product line

BioReagent

form

liquid

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

density

1.077 g/mL at 25 °C

shipped in

ambient

storage temp.

room temp

Application

Used to create a density medium for the purification of lymphocytes and other mononuclear cells. Histopaque®-1077 is a solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/ml. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell mediated lympholysis and for human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step prior to enumeration of T-, B-, and ‘null′ lymphocytes.

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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