L0636
Lectin from Triticum vulgaris (wheat)
BioReagent, suitable for cell culture, lyophilized powder
Synonym(s):
WGA, Wheat germ agglutinin
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About This Item
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sterility
aseptically processed
Quality Level
product line
BioReagent
form
lyophilized powder
technique(s)
cell culture | mammalian: suitable
impurities
salt, essentially free
storage temp.
2-8°C
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General description
Lectin from Triticum vulgaris (wheat) is a dimeric glycoprotein of 36 kDa. It belongs to the family of chitin-binding proteins. Glycosylated precursor of lectin is synthesized in endoplasmic reticulum.
Application
Lectin from Triticum vulgaris (wheat) has been used:
- to conjugate with N-hydroxysuccinimide (NHS) dye
- to inhibit nuclear import
- to inhibit the nuclear pore of human cervical carcinoma HeLa and SiHa cells
Biochem/physiol Actions
Lectin from Triticum vulgaris (wheat) possessing four binding sites is specific for N-acetyl-d-glucosamine and N-acetyl-d-neuraminic acid (sialic acid) residues. Lectins also function as a fungicide. They also possess anti-nutritive effects.
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Other Notes
Highly purified
Preparation Note
Conjugates are prepared from affinity purified lectin.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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