GE17-0756-05
Glutathione Sepharose™ 4B
Cytiva 17-0756-05, pack of 100 mL
Synonym(s):
affinity chromatography resin, GST-tagged proteins purification
About This Item
Recommended Products
ligand
glutathione
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-0756-05
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
4-13
working range
4-13
capacity
>25 mg binding capacity (horse liver GST/ml medium)
General description
GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.
Features and Benefits
- High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
- Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.
This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
Protocols
This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
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