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GE10600029

Amersham™ Hybond® P Western blotting membranes, PVDF

pore size 0.45 μm, roll W × L 150 mm × 4 m, pkg of 1 ea

Synonym(s):

PVDF Blotting Membrane

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About This Item

UNSPSC Code:
41105339
NACRES:
NB.22

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 10600029

roll W × L

150 mm × 4 m

pore size

0.45 μm pore size

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General description

PVDF membranes have higher mechanical strength than NC membranes and, thus, are more suitable for Western blotting applications where stripping and reprobing is needed. In addition, PVDF membranes exhibit higher protein binding capacity, however, with a tendency for a higher background, compared with NC membranes.

Features and Benefits

  • Offers high physical strength.
  • For use with standard colorimetric and chemiluminescent detection methods for proteins of > Mr 20 000.
  • Offers protein binding capacity over 200 μg/cm2.
  • Even after rigorous washing processes, high protein retention is achieved.
  • Sample loss is reduced by high protein capture during transfer.
  • For laboratory use, it is available in a variety of precut sizes.
  • Suitable for stripping and reprobing

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Legal Information

Amersham is a trademark of Cytiva
Hybond is a registered trademark of Cytiva

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Troubleshooting Detection Methods for GST-tagged Proteins

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

Protocols

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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