F7253

Fast Black K Salt hemi(zinc chloride) salt

practical grade

• Synonym(s): 2,5-Dimethoxy-4-([4-nitrophenyl]azo)benzenediazonium chloride hemi(zinc chloride) salt, Brentamine fast black K salt
• Linear Formula: C₁₄H₁₂N₅O₄ · 1/2ZnCl₄
• CAS Number: 64071-86-9
• Molecular Weight: 417.88
• Colour Index Number: 37190
• EC Number: 264-656-1
• MDL number: MFCD00078471
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894935
• NACRES: NA.47

Properties

• grade: practical grade
• Quality Level: 200
• form: powder
• composition: Dye content, ≥25%
• color: brown-red
• mp: 150 °C
• solubility: water: 10 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1N=Nc2ccc(cc2)[N+]([O-])=O.COc3cc([N+]#N)c(OC)cc3N=Nc4ccc(cc4)[N+]([O-])=O
• InChI: 1S/2C14H12N5O4.4ClH.Zn/c2*1-22-13-8-12(14(23-2)7-11(13)16-15)18-17-9-3-5-10(6-4-9)19(20)21;;;;;/h2*3-8H,1-2H3;4*1H;/q2*+1;;;;;+2/p-4
• InChI key: NZWZSUSPTZUGNN-UHFFFAOYSA-J

Description

General description

Fast black K (C.l. 37190) is a lipophilic amino-substituted monoazo dye. It is also known as Brentamine fast black K salt.

Application

Fast Black K Salt hemi (zinc chloride) salt has been used for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE. It has been used for staining endopeptidase and leucine aminopeptidase activity.

Fast black K salt has various applications as listed below-

• to stain enterochromaffin cells > as an indirect stain for periodate-generated tissue aldehyde groups
• as a visualization agent for amphetamines and cyanobacterial toxins following their separation by thin-layer chromatography
• for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE
• staining endopeptidase and leucine aminopeptidase activity
  

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves