F2266
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–FITC antibody produced in sheep
affinity isolated antibody, buffered aqueous solution
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About This Item
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biological source
sheep
Quality Level
conjugate
FITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct immunofluorescence: 1:128
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
IgGs are glycoprotein antibodies that regulate immunological defense mechanisms such as phagocytosis and inflammatory responses . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibodies are specific for mouse IgG subclasses G1, G2a, G2b, and G3 and do not react with serum proteins in rat.
Immunogen
Mouse IgG
Application
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibody is suitable for use in flow cytometry, immunohistochemistry, indirect immunofluorescence, and immunohistochemistry .
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Other Notes
Antibody adsorbed with rat serum proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background staining with rat samples.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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M C Gómez et al.
Reproduction in domestic animals = Zuchthygiene, 47 Suppl 6, 130-135 (2013-01-04)
Somatic cell nuclear transfer offers the possibility of preserving endangered species including the black-footed cat, which is threatened with extinction. The effectiveness and efficiency of somatic cell nuclear transfer (SCNT) depends on a variety of factors, but 'inappropriate epigenetic reprogramming
P Robin et al.
Cell motility and the cytoskeleton, 28(1), 34-44 (1994-01-01)
The role of microtubules in the exocrine secretory process is not yet well established, and their disruption by anti-microtubule drugs leads to variable effects on intracellular transit and protein secretion. We investigated the involvement of microtubules in the regulated secretory
Y Yan et al.
Journal of immunology (Baltimore, Md. : 1950), 166(8), 5258-5264 (2001-04-06)
Donor leukocytes play a dual role in rejection and acceptance of transplanted organs. They provide the major stimulus for rejection, and their removal from the transplanted organ prolongs its survival. Paradoxically, administration of donor leukocytes also prolongs allograft survival provided
C M Newman et al.
Arteriosclerosis, thrombosis, and vascular biology, 15(11), 2010-2018 (1995-11-01)
Osteopontin (OP) is a secreted glycoprotein that contains the Arg-Gly-Asp (RGD) cell-binding sequence that binds calcium and is chemotactic and adhesive for rat vascular smooth muscle cells (VSMCs). OP gene expression is upregulated in cultured rat VSMCs in vitro and
Sambit K Nanda et al.
The Journal of experimental medicine, 208(6), 1215-1228 (2011-05-25)
The protein ABIN1 possesses a polyubiquitin-binding domain homologous to that present in nuclear factor κB (NF-κB) essential modulator (NEMO), a component of the inhibitor of NF-κB (IκB) kinase (IKK) complex. To address the physiological significance of polyubiquitin binding, we generated
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