E0778
Ephrin-B2/Fc Chimera from mouse
>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder
Synonym(s):
ELF-2, Htk-L, LERK-5, NLERK-1
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About This Item
Recommended Products
biological source
mouse
Quality Level
recombinant
expressed in NSO cells
Assay
>90% (SDS-PAGE)
form
lyophilized powder
mol wt
monomer calculated mol wt 49.6 kDa
60-65 kDa by SDS-PAGE (reducing)
packaging
pkg of 200 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Efnb2(13642)
Biochem/physiol Actions
Member of ephrin ligand family that binds Eph receptor tyrosine kinases (EphA4, EphB1, EphB2, EphB3, and EphB4). Involved in pattern formation and morphogenesis. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.
Other Notes
Extracellular domain of mouse ephrin-B2 (amino acids 1-227) fused to the C-terminal 6X histidine-tagged Fc region of human IgG1.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.
Analysis Note
The biological activity is measured by its ability to bind immobilized recombinant mouse EphB2/Fc in ELISA.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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PloS one, 12(7), e0180785-e0180785 (2017-07-08)
We previously reported that EphA4, a member of the Eph family of receptor tyrosine kinases, is an important modulator of growth hormone (GH) signaling, leading to augmented synthesis of insulin-like growth factor 1 (IGF1) for postnatal body growth. In the
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