D1319

Anti-DAP Kinase 1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-DAPK, Anti-DAPK1, Anti-Death associated protein kinase 1
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~160 kDa
• species reactivity: mouse (predicted), human, rat (predicted)
• concentration: ~1 mg/mL
• technique(s): immunoprecipitation (IP): 2.5-5.0 μg using extracts of human HeLa cells; western blot: 1-2 μg/mL using whole extracts of human HeLa cells
• UniProt accession no.: P53355
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DAPK1(1612) ; mouse ... Dapk1(69635) ; rat ... Dapk1(306722)

Description

General description

DAP-kinase 1 is a Ca²⁺/calmodulin dependent Ser/Thr kinase that associates with microfilaments. The protein is composed of a multidomain structure. It has a subdomain typical of serine/threonine kinases, a Ca²⁺/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. It contains two auto-inhibitory domains, one being Ca²⁺/calmodulin dependent.

Specificity

Anti-DAPK1 antibody is specific for human DAPK1 (approx. 160 kDa). In immunoblotting, detection of the DAPK1 band is specifically inhibited with the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 2-13 of human DAP-kinase 1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Application

Anti-DAP Kinase 1 antibody is suitable for use in immunoprecipitation (2.5-5.0 μg using extracts of human HeLa cells) and western blot (1-2 μg/mL using whole extracts of human HeLa cells).

Anti-DAP Kinase 1 antibody produced in rabbit has been used in immunoprecipitation and western blotting.

Biochem/physiol Actions

Death-associated protein kinase (DAPK) belongs to the serine/threonine kinase family that regulates apoptosis, growth factor signaling and also may be involved in modulating immune responses. DAPK1 mediates cellular functions such as membrane blebbing and autophagy and inhibits the activity of prolyl isomerase

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves