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product name
MEF-1, 98061101
biological source
mouse embryo
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse embryo fibroblast, SV40 - transformed
Cell Line Description
The cell line MEF-1 was established by transfection of mouse embryo fibroblasts ((C57 BL/6 x 129) F1) with a SV40 coding plasmid. MEF-1 express the wild-type for low density lipoprotein receptor related protein (LRP). Together with the cell lines PEA10 (Sigma Catalogue number. 97040310) and PEA13 (Sigma Catalogue number. 97040311), which express heterozygous and homozygous forms of LPR, an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LPR - deficiency. This is of importance since LRP - deficient mouse embryos die early during gestation.
Application
Study of LRP and LRP-deficiency
Culture Medium
DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:6 to 1:10 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Other Notes
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Certificates of Analysis (COA)
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