Vero (AC-free)
8011101, African green monkey kidney, Epithelial
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About This Item
UNSPSC Code:
41106514
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product name
Vero (AC-free), from African green monkey kidney, 08011101, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
biological source
African green monkey kidney
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
receptors
Not specified
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General description
Vero cells are anchorage-dependent cells derived from the kidney of African green monkey cells. They have applications in molecular and cell biology research. Vero cells are a useful host for the cultivation of Rabies virus, Reovirus and Japanese encephalitis virus.
Cell Line Origin
African green monkey kidney cells, serum-free
Cell Line Description
Vero cell line (Sigma catalog no. 84113001) adapted to grow in animal component free medium (VP-SFM; Gibco catalog no. 11681-020).
Culture Medium
VP-SFM (Gibco cat no. 11681-020) + 4 mM L-Glutamine (G7513). When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO (D2650).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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Certificates of Analysis (COA)
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