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Key Documents

A5979

Sigma-Aldrich

Anti-ARP3 antibody, Mouse monoclonal

clone FMS338, purified from hybridoma cell culture

Synonym(s):

ARP3 Detection Antibody, Mouse Anti-ARP3

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FMS338, monoclonal

form

buffered aqueous solution

mol wt

antigen 40-45 kDa

species reactivity

human, mouse, rat, canine

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of mouse myoblast C2 cells

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTR3(10096)
mouse ... Actr3(74117)
rat ... Actr3(81732)

General description

Monoclonal Anti-ARP3 (mouse IgG2b isotype) is derived from the hybridoma FMS338 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant human ARP3. Arp3 is mapped to human chromosome 2q14.1.

Immunogen

recombinant human ARP3.

Application

Monoclonal Anti-ARP3 antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions

The ARP2/3 complex consists of seven distinct proteins (Arp2 and Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc) that modulate polymerization of cellular actin filaments. ARP3 regulates actin nucleation, and is involved in directing the movement of Listeria monocytogenes in infected host cells. Monoclonal Anti-ARP3 antibody binds to human, canine, rat, and mouse ARP3.
The actin-related protein-2/3 (ARP2/3) is part of Wiskott-Aldrich Syndrome protein (WASP) signaling in mediating polymerization state of actin filaments. It has been found that ARP2/3 complex and its activator spinocerebellar ataxia, autosomal recessive 2 (Scar2) are involved in Golgi polarization in NIH 3T3 cells. However in primary astrocytes, Golgi polarization involves neither actin cytoskeleton or Arp2/3 complex nor any WASP-family of proteins. In a different model, it was found that the ARP2/3 complex was necessary for neutrophil chemotaxis and phagocytosis. By genetic and loss of function studies, it was demonstrated that Scar2 is the major regulator of the ARP2/3 complex rather than the WASP family of proteins, the latter having more restricted roles in specific cellular events.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pablo Vargas et al.
Nature cell biology, 18(1), 43-53 (2015-12-08)
Dendritic cell (DC) migration in peripheral tissues serves two main functions: antigen sampling by immature DCs, and chemokine-guided migration towards lymphatic vessels (LVs) on maturation. These migratory events determine the efficiency of the adaptive immune response. Their regulation by the
Ying Gao et al.
Scientific reports, 7(1), 1110-1110 (2017-04-26)
PFOS (perfluorooctanesulfonate, or perfluorooctane sulfonic acid) is an anthropogenic fluorosurfactant widely used in consumer products. While its use in Europe, Canada and the U.S. has been banned due to its human toxicity, it continues to be used in China and
Arp2/3 and VASP are essential for fear memory formation in lateral amygdala
Basu S, et al.
eNeuro, 3, 1279-1291 (2016)
Nan Li et al.
Spermatogenesis, 6(1), e1206353-e1206353 (2016-08-26)
Throughout the epithelial cycle of spermatogenesis, actin microfilaments arranged as bundles near the Sertoli cell plasma membrane at the Sertoli cell-cell interface that constitute the blood-testis barrier (BTB) undergo extensive re-organization by converting between bundled and unbundled/branched configuration to give
Environmental toxicants perturb human Sertoli cell adhesive function via changes in F-actin organization mediated by actin regulatory proteins
Xiao X, et al.
Human Reproduction, 29(6), 1279-1291 (2014)

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