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Key Documents

93290

Sigma-Aldrich

TRIS borate – EDTA buffer solution

for Northern and Southern blotting, solution

Synonym(s):

TBE buffer

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About This Item

UNSPSC Code:
41105319
eCl@ss:
32129211
NACRES:
NA.25

grade

for molecular biology

Quality Level

100

description

long run, concentrate

product line

BioReagent

form

solution

quality

10× concentrate
ready-to-use solution

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

8.6-9.0

storage temp.

room temp

Related Categories

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. The TBE solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

Components

The TBE 10x liquid contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water.

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Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H360FD

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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L. Johnston-Dow et al.
Biotechniques, 5, 754-754 (1987)

Protocols

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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