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Sigma-Aldrich

Atto 565-Streptavidin

BioReagent, suitable for fluorescence

Synonym(s):

Streptavidin-Atto 565

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About This Item

MDL number:
MFCD04040676
UNSPSC Code:
12352108
NACRES:
NA.32

product line

BioReagent

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 565 nm; λem 590 nm in 0.1 M phosphate pH 7.0

suitability

corresponds for gel electrophoresis
suitable for fluorescence

storage temp.

−20°C

Application

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). It does nearly not show cis trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Streptavidin conjugation of Atto 565 does not significantly change the labels spectral data regarding excitation and emission maxima. Atto 565-Streptavidin is used to label biotin conjugated molecules.

Analysis Note

free of unconjugated dye

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lukasz Krzemiński et al.
The journal of physical chemistry. B, 115(43), 12607-12614 (2011-09-24)
Recently, studies have been reported in which fluorescently labeled redox proteins have been studied with a combination of spectroscopy and electrochemistry. In order to understand the effect of the dye on the protein-electrode interaction, voltammetry and surface analysis have been
Judith E Berlier et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(12), 1699-1712 (2003-11-19)
Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm, 700 nm, and 750 nm were conjugated to antibodies and other selected proteins. These conjugates were
PALM and STORM. Unlocking live-cell super-resolution.
Henriques, R.; Griffiths, C.; Hesper Rego, E.; Mhlanga, Musa M.
Biopolymers, 95(5), 322-331 (2011)
Direct stochastic optical reconstruction microscopy with standard fluorescent probes.
van de Linde, S.; et al.
Nature Protocols, 6(7), 991-1009 (2011)

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