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5008

Sigma-Aldrich

Human Collagen Type I & III

from neo-natal fibroblast cells, 97% Type I with the remainder of Type III collagen, powder, suitable for cell culture, VitroCol®

Synonym(s):

Collagen Solution

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About This Item

EC Number:
232-697-4
UNSPSC Code:
12352202
NACRES:
NA.77

product name

VitroCol®, Collagen from Human neonatal fibroblasts, 97% Type I with the remainder of Type III collagen, suitable for cell culture

Quality Level

100

sterility

sterile-filtered

form

lyophilized

packaging

pkg of 15 mg

manufacturer/tradename

Advanced BioMatrix

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/mL Endotoxin level

shipped in

wet ice

storage temp.

−20°C

General description

VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblasts to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.

VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.

VitroCol® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. It is not recommend for the formation of a solid gel. VitroCol® is supplied as a sterile, lyophilized powder.

Preparation Note

This product is supplied as a lyophilized powder with 15 mg of human collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Legal Information

VitroCol is a registered trademark of Advanced BioMatrix, Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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