04022

Atto 665 NHS ester

BioReagent, suitable for fluorescence

• UNSPSC Code: 12352108
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 100
• Assay: ≥90% (coupling to amines)
• mol wt: M_w 820 g/mol
• manufacturer/tradename: ATTO-TEC GmbH
• fluorescence: λ_ex 663 nm; λ_em 684 nm in 0.1 M phosphate pH 7.0
• suitability: suitable for fluorescence
• storage temp.: −20°C

Description

General description

Atto 665 is a new fluorescent label closely related to Atto 647N. It shows extraordinary high fluorescence quantum yield, excellent thermal and photo-stability, outstanding ozone resistance, and very little triplet formation. Atto 665 is a cationic (charge ⁺¹). The dye is moderately hydrophilic. The NHS-ester of the dye reacts easily with amino-groups of proteins and other bio-molecules. Since the amino group must be non protonated to be reactive, the pH of the reaction is recommended to be about 8.3. Prepare labeling solution of NHS-ester immediately before use by dissolving the vial content in anhydrous and amine-free DMF or DMSO.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable