E6003

Eosin Y disodium salt

Dye content ≥85%, Powder

• Synonym(s): 2′,4′,5′,7′-Tetrabromofluorescein disodium salt, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, D&C;Red No. 22, Eosin yellowish
• Empirical Formula (Hill Notation): C₂₀H₆Br₄Na₂O₅
• CAS Number: 17372-87-1
• Molecular Weight: 691.85
• Colour Index Number: 45380
• Beilstein: 3586809
• EC Number: 241-409-6
• MDL number: MFCD00005040
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894349
• NACRES: NA.47

Properties

• product name: Eosin Y disodium salt, Dye content ≥85 %
• form: powder
• Quality Level: 200
• composition: Dye content, ≥85%
• technique(s): titration: suitable
• color: brown
• pH: 6.55 (29 °C, 84 °F)
• mp: 255 — 270 °C (491 - 518 °F at 977.9 hPa)
• solubility: H₂O: 1 mg/mL
• density: 1.018 g/cm³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
• InChI: 1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• InChI key: SEACYXSIPDVVMV-UHFFFAOYSA-L

Description

Application

Eosin Y disodium salt has been used for determining sperm viability. The swollen and stained spermatozoa were considered dead. In corneal epithelium, it has been used in the procedure of immunohistological staining.

Biochem/physiol Actions

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317,H319
• Precautionary Statements: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): 376.9 °F - Pensky-Martens closed cup
• Flash Point(C): 191.6 °C - Pensky-Martens closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves