A92775

Arsenazo III

calcium-sensitive dye

• Synonym(s): 2,2′-(1,8-Dihydroxy-3,6-disulfonaphthylene-2,7-bisazo)bisbenzenearsonic acid, 2,7-Bis(2-arsonophenylazo)chromotropic acid
• Linear Formula: (HO)₂C₁₀H₂(SO₃H)₂(N=NC₆H₄AsO₃H₂)₂
• CAS Number: 1668-00-4
• Molecular Weight: 776.37
• Beilstein: 5717957
• EC Number: 216-788-6
• MDL number: MFCD00036695
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891431
• NACRES: NA.47

Properties

• Quality Level: 200
• form: powder
• technique(s): titration: suitable
• impurities: <0.1% Ca
• color: brown to very dark brown, and Black
• mp: >320 °C (lit.)
• solubility: ammonium hydroxide: 1 mg/mL
• λmax: 530 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Oc1c(\N=N\c2ccccc2[As](O)(O)=O)c(cc3cc(c(\N=N\c4ccccc4[As](O)(O)=O)c(O)c13)S(O)(=O)=O)S(O)(=O)=O
• InChI: 1S/C22H18As2N4O14S2/c29-21-18-11(9-16(43(37,38)39)19(21)27-25-14-7-3-1-5-12(14)23(31,32)33)10-17(44(40,41)42)20(22(18)30)28-26-15-8-4-2-6-13(15)24(34,35)36/h1-10,29-30H,(H2,31,32,33)(H2,34,35,36)(H,37,38,39)(H,40,41,42)/b27-25+,28-26+
• InChI key: UQHVTNUJRKELCE-NBHCHVEOSA-N

Description

Application

Arsenazo III has been used as a color forming reagent to determine uranium concentration.

Biochem/physiol Actions

Arsenazo III is a metallochromic dye. Arsenazo III is used in the determination of calcium in biological samples. It is used to evaluate calcium transport in permeabilized cells. It is also used for the detection of rare earth metals (polyvalent metal ions). Arsenazo III is used for the spectrophotometric determination of uranium and thorium.

Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H301 + H331 - H410
• Precautionary Statements: P261 - P264 - P270 - P273 - P301 + P310 - P304 + P340 + P311
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves