85766

m-Endo Agar LES

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Membrane Endo Agar LES
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; casein hydrolysate, 3.7 g/L ; di-potassium hydrogen phosphate, 3.3 g/L ; lactose, 9.4 g/L ; pararosanilin (fuchsin), 0.8 g/L ; peptone from meat, 3.7 g/L ; potassium hydrogen phosphate, 1 g/L ; sodium chloride, 3.7 g/L ; sodium deoxycholate, 0.1 g/L ; sodium lauryl sulfate, 0.05 g/L ; sodium sulfite, 1.6 g/L ; tryptose, 7.5 g/L ; yeast extract, 1.2 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.2±0.2±25 °C
• application(s): agriculture; bioburden testing; environmental; food and beverages; water monitoring; microbiology
• suitability: selective and differential for Escherichia coli; selective and differential for Proteus spp.; selective and differential for Salmonella spp.; selective and differential for coliforms

Description

Application

m-Endo Agar LES can be used for the enumeration of coliforms in water using the Membrane filter technique for coliform enumeration. This method is more reliable and precise than MPN multiple test. It follows the two step membrane filter procedure using Lauryl Sulfate Broth (Cat. No. 17349) as a preliminary enrichment, resulting in higher coliform counts.

Preparation Note

Suspend 51 g in 1 litre of distilled water containing 20 ml of ethanol 96 %. Boil to dissolve completely. Do not autoclave! Cool to 45-50°C and dispense 4 ml amounts into 50-60 mm Petri dishes and allow to solidify. The agar should be at least 1.5mm depth. Do not expose plates to direct sunlight.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H350
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves