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69964

Millipore

MRS Agar

suitable for microbiology, NutriSelect® Basic

Synonym(s):

Lactobacillus Agar according to DeMan, Rogosa and Sharpe

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About This Item

UNSPSC Code:
41171605
NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12 g/L
dipotassium hydrogen phosphate, 2 g/L
D(+)-glucose, 20 g/L
magnesium sulfate (equivalent to 0.2 g/lheptahydrate), 0.1 g/L
manganous sulfate monhydrate, 0.05 g/L
meat extract, 5 g/L
sodium acetate, 5 g/L
triammonium citrate, 2 g/L
universal peptone, 10 g/L
yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

6.5±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages

microbiology

suitability

nonselective for Lactobacillus spp.
selective by low pH for Bifidobacterium spp.

Application

For the enrichment, cultivation and isolation of all species of Lactobacillus from all types of material

Preparation Note

Suspend 61.15 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN® 80; Sigma P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Hanan R Shehata et al.
Frontiers in microbiology, 11, 1095-1095 (2020-06-26)
Probiotics are defined as "live microorganisms that, when administered in adequate amounts, confer a health benefit on the host." The diverse health benefits have contributed to rapid increase in probiotic consumption and in the value of probiotic market, valued at
J.D. DeMan et al.
The Journal of Applied Bacteriology, 23, 130-130 (1960)
Eleftheria Barouni et al.
Food chemistry, 200, 76-82 (2016-02-03)
The object of the current study was to develop a new process for continuous Feta-type cheese production using a biocatalyst consisting of immobilized rennin on a tubular cellulose/starch gel (TC/SG) composite, which has been proven to be an appropriate carrier
Marie-Paule Nawrot-Esposito et al.
Insects, 11(10) (2020-10-18)
Bioinsecticides made from the bacterium Bacillus thuringiensis (Bt) are the bestselling bioinsecticide worldwide. Among Bt bioinsecticides, those based on the strain Bt subsp. kurstaki (Btk) are widely used in farming to specifically control pest lepidopteran larvae. Although there is much
Rupal Mistry et al.
Genetics, 206(2), 889-904 (2017-04-18)
Resident gut bacteria are constantly influencing the immune system, yet the role of the immune system in shaping microbiota composition during an organism's life span has remained unclear. Experiments in mice have been inconclusive due to differences in husbandry schemes

Articles

Molecular biology-based methods, like PCR, can be used for lactobacilli detection. However, they are often quite expensive. We provide a revolutionary molecular biology method that is rapid, easy, and cost-effective.

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

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