SCR006
Accumax™Cell Counting Solution in DPBS Sterile-filtered Cell Culture Tested
A cell detachment solution of proteolytic, collagenolytic & DNAse enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.
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About This Item
UNSPSC Code:
12352204
eCl@ss:
32160801
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Quality Level
form
liquid
manufacturer/tradename
Chemicon®
input
sample type mesenchymal stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type cardiac stem cell(s)
sample type: human embryonic stem cell(s)
sample type pancreatic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
shipped in
dry ice
Related Categories
General description
A cell detachment solution of proteolytic, collagenolytic and DNAse enzymes. Useful on a variety of cells lines however not all cells or cell types are affected by ACCUMAX™, also passage number and confluency can influence how well the reagent does because the amounts and makeup of the collagen extracellular matrix will vary with age and culture state. Because ACCUMAX is a more gentle, less univerally proteolytic cleavage mixture reagent amounts and incubation times need to be optimized for each cell system.
The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlastic. ACCUMAX does not contain mammalian or bacterial derived products.
Each lot of ACCUMAX is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.
ACCUMAX has been used successfully on numerous cell types including: CHO, BHK, 293, COS, Sf9, and catfish primary hepatocyte cells.
The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlastic. ACCUMAX does not contain mammalian or bacterial derived products.
Each lot of ACCUMAX is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.
ACCUMAX has been used successfully on numerous cell types including: CHO, BHK, 293, COS, Sf9, and catfish primary hepatocyte cells.
Application
A cell detachment solution of proteolytic, collagenolytic & DNAse enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.
Physical form
100 ml, ready to use, frozen sterile liquid. 1X ACCUMAX™ enzymes in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5mM EDTA-4Na.
Storage and Stability
Stable when stored at -20°C. Refer to lot expiration date. Recommended storage upon receipt is -20°C. After thawing, ACCUMAX may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMP. Use sterile technique when handling.
Legal Information
Accumax is a trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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