SCC222

Mouse mammary tumor cell line TUBO

• Synonym(s): Turin-Bologna cell line;Mouse mammary tumor cell line;Murine mammary tumor cells;Murine mammary cancer cell line
• UNSPSC Code: 41106514

Properties

• biological source: mouse
• Quality Level: 100
• packaging: vial of ≥1X10⁶ cells
• manufacturer/tradename: Millipore
• growth mode: N/A
• description: (≥1x10⁶ viable TUBO Mouse Mammary Tumor Cells: (Catalog No. SCC222). Store in liquid nitrogen. )
• technique(s): cell based assay: suitable; cell culture | mammalian: suitable
• shipped in: liquid nitrogen
• storage temp.: −196°C

Description

Application

• Each vial contains > 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for interspecies contamination from human, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.

Breast cancer is the most common malignancy in women throughout the world. The global incidence rate has been increasing annually at a rate of 3.1%. Globally, about 2.1 million women were diagnosed with new occurrences of breast cancer and 626,679 women died with breast cancer in 2018. Higher reported incidence rates in wealthier regions may be explained by enhanced access to medical care and diagnostic technologies. Early diagnosis often results in a more positive prognosis.

HER2/neu is an important gene implicated in various cancer types, but particularly in breast cancer. It is a part of the epidermal growth factor receptor family (EGFR). Its protein product is a p185 tyrosine kinase growth factor receptor that is homologous to other EGFRs. When overexpressed or mutated, the extracellular domain will often form homodimers or heterodimers with other EGFRs. Overexpression has typically been correlated with poorer prognosis, higher invasiveness, and can be used to predict the response of certain treatments. HER2/neu gene amplification or overexpression is present in 25-30% of invasive breast carcinomas. The involvement of p185 in tumor progression and initiation has therefore made it a target of interest for therapeutics.

Source
The TUBO line is a cloned cell line that was established in vitro from a lobular carcinoma that arose spontaneously in a BALB-neuT mouse.

References
1.J Immunol 2000,165(9): 5133-5142.
2.Nat Rev Dis Primers 2019, 5(1): 66.
3.Prog Mol Biol Transl Sci. 2018, 160: xi-xii. doi: 10.1016/S1877-1173(18)30143-1.

Features and Benefits

Rat p185 is xenogeneic in BALB/c mice, differing in <lt/>6% of the amino residues from mouse p185.1 In the rat-p185 protein, a single point mutation results in the transformation of the Her-2/neu protooncogene to a dominant oncogene. The TUBO cell line has also been shown to express MHC class I glycoproteins, but not MHC class II. The use of the TUBO cell line enables further research into the mechanisms of breast cancer development and potential for new therapies or treatments.

Storage and Stability

TUBO cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable