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Key Documents

MAC115

Sigma-Aldrich

Anti-human Albumin Antibody, clone 8F6F9

ascites fluid, clone 8F6F9, from mouse

Synonym(s):

Serum albumin, ALB, human albumin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

8F6F9, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ALB(213)

General description

Human Albumin, also known as Serum albumin (ALB), is a member of the ALB/AFP/VDB family. Serum albumin is the main protein of plasma and has been shown to bind with water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Human Albumin plays an important role in the regulation of the colloidal osmotic pressure of the blood. Additionally, Human Albumin has been shown to be the major zinc transporter in plasma, and typically binds about 80% of all plasma zinc. Mutations in ALB are implied to be the cause of familial dysalbuminemic hyperthyroxinemia (FDH), a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T4. Human Albumin is expressed primarily in plasma.

Immunogen

Human sera albumin

Application

Detect Albumin using this mouse monoclonal antibody, Anti-human Albumin Antibody, clone 8F6F9 validated for use in western blotting.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in human serum and plasma.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected human Albumin in human serum and plasma.

Optimal working dilutions must be determined by end user.

Target description

~50 and ~69 observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Human serum and plasma

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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