HPLX1-115SP-PX

MILLIPLEX^® PLEXpedition Screening Premixed Kits (80-plex & 115-plex)

• Synonym(s): Human screening kit, Human screening panel, Luminex human screening immunoassay, Luminex human screening panel, Millipore screening kit, Millipore screening panel, Multiplex screening kit, Multiplex screening panel
• UNSPSC Code: 12161503

Properties

• description: 96 well
• Quality Level: 100
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• parameter: 25 μL sample volume (per well)
• assay range: intra-assay cv: ≤15%; inter-assay cv: ≤20%; (≤25% for PDGF-AA)
• technique(s): multiplexing: suitable using : Suitable
• input: cell culture supernatant(s); serum; plasma
• detection method: fluorometric (Luminex^® xMAP^®)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The MILLIPLEX^® PLEXpedition Screening Panel is a configurable multiplex immunoassay kit developed to simultaneously screen up to 115 targets including cytokines, chemokines, growth factors, matrix metalloproteinases (MMPs), and biomarkers of bone health, metabolism, and cardiovascular disease. Two Luminex^® instruments, the xMAP^® INTELLIFLEX and the FLEXMAP 3D^®, can analyze all 115 bead regions with comparable sensitivity and sample correlation while the MAGPIX^® and Luminex 200^™ instruments can detect specific subsets of 50 and 80 analytes, respectively. As a discovery biomarker tool this panel is provided in a matrix-free format to enable analyte detection in multiple sample types.

Specificity

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel, with the following exceptions:

• GIP and ENA-78 have low cross-reactivity with IL-23 (≤15%) and GRO (≤5%) recombinant proteins, respectively.
• IL-12 (p40) has moderate, but expected, cross-reactivity with the recombinant proteins for IL-12 (p70) and IL-23 which both contain the p40 subunit.
• MMP-3 may exhibit significant cross-reactivity with MMP-10 recombinant proteins due to high sequence homology, however, analysis demonstrated independent and differentiated measurement of MMP-3 and MMP-10 in biological samples.

Application

The MILLIPLEX^® PLEXpedition Magnetic Bead Screening Panel is to be used for the simultaneous quantification of up to 115 analytes in your selected sample type. (NOTE: Instrument requirements vary based on analyte selection.) This is an overnight assay and requires 25 µL per well of neat or diluted sample.

• MAGPIX^®/Luminex 200^™/FLEXMAP 3D^®/xMAP^® INTELLIFLEX required for any or all of the following 50 analytes: EGF, FGF-2, Fractalkine, G-CSF, Ghrelin (Active), GIP, Glucagon, GM-CSF, Granzyme B, GROα, HGF, IFNα2, IFNγ, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-18, IL-1Ra, IL-1α, IL-1β, IL-2, IL-22, IL-23, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, Leptin, MCP-1, MIG, MIP-1α, MIP-1β, OPN, PDGF-AA, PDGF-BB, Perforin, RANTES, sCD40L, SCF, sFAS, sFASL, TGFα, TNFα, TRAIL, VEGF-A
• Luminex 200^™/FLEXMAP 3D^®/xMAP^® INTELLIFLEX required for any or all of the following 80 analytes: ACTH, BDNF, C-Peptide, EGF, Eotaxin, FABP4, FGF-2, FLT3 Ligand, Fractalkine, G-CSF, Ghrelin (Active), GIP, GLP-1 Total, Glucagon, GM-CSF, Granzyme A, Granzyme B, GROα, HGF, IFNα2, IFNγ, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-18, IL-1Ra, IL-1α, IL-1β, IL-2, IL-21, IL-22, IL-23, IL-3, IL-33, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, Insulin, IP-10, I-TAC, Leptin, MCP-1, MCP-3, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-3α, MMP-1, MMP-2, MMP-7, MMP-9, OPG, OPN, PDGF-AA, PDGF-BB, Perforin, PlGF, PP, PYY, RANTES, sCD40L, SCF, sFAS, sFASL, TARC, TGFα, TNFα, TNFβ, TRAIL, Troponin I, VEGF-A
• FLEXMAP 3D^®/xMAP^® INTELLIFLEX required for any or all of the following 115 analytes: ACTH, Amylin (Active), BCA-1, BDNF, BNP, CK-MB, C-Peptide, DKK1, EGF, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, Erythropoeitin (EPO), FABP3, FABP4, FGF-2, FGF-21, FGF-23, FLT3 Ligand, Fractalkine, G-CSF, Ghrelin (Active), GIP, GLP-1 Total, Glucagon, GM-CSF, GPC-2, Granzyme A, Granzyme B, GROα, HB-EGF, HGF, I-309, IFNα2, IFNγ, IL-10, IL-11, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-18, IL-1Ra, IL-1α, IL-1β, IL-2, IL-21, IL-22, IL-23, IL-3, IL-33, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, Insulin, IP-10, I-TAC, Leptin, LIF, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-1Ϭ, MIP-3α, MIP-3β, MMP-1, MMP-10, MMP-12, MMP-2, MMP-3, MMP-7, MMP-9, Myostatin , NGF, NTproBNP, OPG, OPN, Osteocalcin, Osteonectin, PDGF-AA, PDGF-BB, Perforin, PlGF, PP, PYY, RANTES, sCD40L, SCF, SDF-1, sFAS, sFASL, SOST, TARC, TGFα, TNFα, TNFβ, TPO, TRAIL, Troponin I, TSLP, VEGF-A, VEGF-C, VEGF-D

Features and Benefits

This is a configurable kits. Design your multiplex kit by choosing available analytes within this panel based on your instrument.

Legal Information

FLEXMAP 3D system is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

Luminex 200 system is a trademark of Luminex Corp

MAGPIX system is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.