ABE70
Anti-BAF180 Antibody
from rabbit
Synonym(s):
polybromo 1, protein polybromo-1, BRG1-associated factor 180, polybromo-1D, Polybromo-1D
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, rat
technique(s)
ChIP: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PBRM1(55193)
General description
BRG1-associated factor 180 (BAF180) is a subunit of the SWI/SNF-B (PBAF) chromatin remodeling complex, and is known to contain 6 bromo domains, 2 BAH domains, and a single HMG box DNA-binding domain. BAF180 has a critical and active role in the transcriptional activation and repression of specific genes via chromatin remodeling, including genes involved in the development of the heart, thus suggesting a role in mammalian development. Recent studies demonstrate BAF180 to suppress tumorigenesis through its ability to regulate both baseline and signal-dependent p21 transcription by its binding of the p21 promoter. Expression of BAF180 is reported in a wide range of tissues.
Immunogen
GST-tagged recombinant protein corresponding to human BAF180.
Application
Anti-BAF180 Antibody is a Rabbit Polyclonal Antibody for detection of BAF180 also known as protein polybromo-1, BRG1-associated factor 180 & has been validated in WB.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP (Xia, W., et al. (2008).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Quality
Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected BAF180 in 10 µg of HEK293 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected BAF180 in 10 µg of HEK293 cell lysate.
Target description
~193 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HEK293 cell lysate
HEK293 cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Darmood Wei et al.
Genes, chromosomes & cancer, 59(8), 472-483 (2020-04-08)
Renal medullary carcinoma (RMC) is a rare, aggressive disease that predominantly afflicts individuals of African or Mediterranean descent with sickle cell trait. RMC comprises 1% of all renal cell carcinoma diagnoses with a median overall survival of 13 months. Patients are
Lucia Daxinger et al.
Genome biology, 14(9), R96-R96 (2013-09-13)
We have used a sensitized ENU mutagenesis screen to produce mouse lines that carry mutations in genes required for epigenetic regulation. We call these lines Modifiers of murine metastable epialleles (Mommes). We report a basic molecular and phenotypic characterization for
Lili Liao et al.
Molecular oncology, 13(4), 811-828 (2018-12-27)
Polybromo-1 (PBRM1) is an important tumor suppressor in kidney cancer. It contains six tandem bromodomains (BDs), which are specialized structures that recognize acetyl-lysine residues. While BD2 has been found to bind acetylated histone H3 lysine 14 (H3K14ac), it is not
Janet Y Leung et al.
PloS one, 7(10), e47967-e47967 (2012-10-20)
The E2F6 protein functions as an Rb-independent repressor of gene transcription. We have previously provided evidence suggesting a role for E2F6 in repression of E2F-responsive genes at S phase. Here, we have identified BRG1, the ATPase subunit of the SWI/SNF
Cornelia Meisenberg et al.
Molecular cell, 73(2), 212-223 (2018-12-18)
Cohesin subunits are frequently mutated in cancer, but how they function as tumor suppressors is unknown. Cohesin mediates sister chromatid cohesion, but this is not always perturbed in cancer cells. Here, we identify a previously unknown role for cohesin. We
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