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Key Documents

ABE2580

Sigma-Aldrich

Anti-Histone H3Kme3Q5Ser

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

antibody form

purified antibody

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

ChIP: suitable
dot blot: suitable
western blot: suitable

isotype

IgG

UniProt accession no.

General description

KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3 trimethylated on lysine 4 and serotonylated on glutamine 5.

Specificity

This rabbit polyclonal antibody specifically detects Histone H3 trimethylated on Lysine 4 and serotonylated on glutamine 5.

Application

Epitope: N-terminus

Quality

Evaluated by Dot Blot on H3(1-10)-control peptide and H3K4me3Q5serotonyl peptide.



Dot Blot Analysis: A 1:500 dilution of this antibody detected Histone H3Kme3Q5Ser peptide, but not the control in H3(1-10) peptide.

Physical properties

~16 kDa observed; 15.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Human, Mouse.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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