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HT96 BL21(DE3) Competent Cells - Novagen
The HT96 BL21(DE3) Competent Cells are designed for high-throughput transformation and protein expression applications.
Synonym(s):
BL21 strain
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About This Item
UNSPSC Code:
41106202
biological source
Escherichia coli
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
transformation efficiency: >2×106 cfu/μg
shipped in
dry ice
storage temp.
2-8°C
Related Categories
General description
Genotype: F-ompT hsdSB(rB- mB-) gal dcm (DE3)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
The HT96 BL21(DE3) Competent Cells are designed for high-throughput transformation and protein expression applications.
The HT96<TMSYMBOL></TMSYMBOL> BL21(DE3) Competent Cells aredesigned for high-throughput transformation.The cells are predispensed in 20-l volumesin a sturdy 96-well polypropylene plate,which is compatible with a variety of thermalcyclers and water baths. Wells are individuallysealed and have raised rims to prevent cross-contamination.Seals can be easily piercedwith standard pipet tips or removed for access.Strips of caps are also provided for reliablesealing during manipulation and storage.Groups of 24 wells can be easily split fromthe plate for processing smaller numbers of samples.
HT96 BL21(DE3) Competent Cells
BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases. The designation (DE3) indicates that the strain is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter.
HT96 BL21(DE3) Competent Cells
BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases. The designation (DE3) indicates that the strain is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter.
Features and Benefits
• High-throughput, 96-well format
Components
1 plate4 plates20 plates
•1 plate4 plates20 platesHT96 BL21(DE3) Competent Cells
•1 × 14 ml4 × 14 ml20 × 14 mlSOC Medium
•1 × 10 µl2 × 10 µl10 × 10 µlTest Plasmid
•1 pkg4 pkg20 pkg8-Cap Strips
•1420Reagent Reservoirs
•1 plate4 plates20 platesHT96 BL21(DE3) Competent Cells
•1 × 14 ml4 × 14 ml20 × 14 mlSOC Medium
•1 × 10 µl2 × 10 µl10 × 10 µlTest Plasmid
•1 pkg4 pkg20 pkg8-Cap Strips
•1420Reagent Reservoirs
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Other Notes
Phillips, T.A., et al. 1984. J. Bacteriol.159, 283.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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