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Key Documents

70796-M

Sigma-Aldrich

His•Tag® Monoclonal Antibody

Novagen®

Synonym(s):

Anti-His antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

antibody form

purified antibody

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Sensitive, specific detection of His•Tag fusion proteins
The HIS TAG® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the HIS TAG® sequence encoded by many of the NOVAGEN® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10-8 to 1 × 10-9 M) enables sensitive, specific detection of HIS TAG® fusion proteins at antibody concentrations of 0.1-0.2 g/ml. The HIS TAG® Monoclonal Antibody binds to N-terminal, C-terminal, and internal HIS TAG® sequences. This antibody also detects the recombinant marker bands in the Perfect Protein<TMSYMBOL></TMSYMBOL> and Trail Mix<TMSYMBOL></TMSYMBOL> Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-g package size provides enough purified antibody for 1000 mL of working solution.

* Not recommended for HRP-based detection of Trail Mix Western Markers.

Warning

Toxicity: Standard Handling (A)

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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