1.52258
Chromolith® WP 300 Protein A (monolithic) HPLC Columns
L × I.D. 25 mm x 4.6 mm HPLC Column
Synonym(s):
Chromolith® WP 300 Protein A 25-4.6 HPLC column
About This Item
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product name
Chromolith® WP 300 Protein A, L × I.D. 25 mm x 4.6 mm HPLC column
material
PEEK column
product line
Chromolith®
feature
endcapped: no
endcapped: no
packaging
1 ea of
parameter
2 - 45 °C temp. range
2-45 °C temp. range
200 bar pressure
200 bar pressure
technique(s)
HPLC: suitable
HPLC: suitable
LC/MS: suitable
column L × I.D.
2.5 cm × 4.6 mm
surface area
120 m2/g
matrix
monolithic silica
monolithic silica
matrix active group
Protein A bonding phase
Protein A bonding phase
pore size
2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity
operating pH range
1.5-7.5
operating pH
1.5-7.5
separation technique
affinity
storage temp.
2-8°C
2-8°C
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General description
Chromolith® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.
Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
Chromolith® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Silica monoliths with larger mesopores are a powerful tool for the fast separation of biomolecules including mAbs. The immobilization of protein A on epoxy-modified widepore (WP) silica rods enabled highly reproducible and fast, one minute, separations of mAbs. The Chromolith® WP 300 is also available with a protein A phase.
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HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
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