1.01301

Auramine O (C.I. 41000)

for microscopy

• Synonym(s): Auramine O (C.I. 41000), Pyoktamin yellow, 4,4′-Bis-(dimethylamino)-benzophenonimide hydrochloride
• Empirical Formula (Hill Notation): C₁₇H₂₂ClN₃
• CAS Number: 2465-27-2
• Molecular Weight: 303.83
• MDL number: MFCD00012484
• UNSPSC Code: 41116130
• EC Index Number: 219-567-2

Properties

• Quality Level: 400
• form: solid
• potency: 1000 mg/kg LD₅₀, oral (Rat)
• pH: 6-7 (20 °C, 10 g/L in H₂O)
• mp: 265 °C (decomposition)
• solubility: 10 g/L
• bulk density: 430 kg/m³
• εmax: 1100-1300 at 431-433 nm at 0.005 g/L (pH 6.0)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
• InChI key: KSCQDDRPFHTIRL-UHFFFAOYSA-N

Description

General description

Auramine O (C.I. 41000)-for microscopy, is a dry dye for fluorescence and can be used acc. to Hagemann-Hermannof for the staining acid-fast bacteria (AFB) in smears and sections of human origin . The mechanism of the fluorescence staining is similar to the classical Ziehl-Neelsen method. The cell wall of AFB has a high proportion of wax and lipids and hence absorbs dyes only very slowly. Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining.
Pretreatment of the specimens with Sputofluol^™ dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol^™ also has a disinfectant effect, with the result that any microorganisms that are present are killed off. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

For microscopy. Staining of mycobacteria according to Hagemann-Hermann, fluorescence staining.
CAS No. 2465-27-2, EC Number 219-567-2.

Analysis Note

Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (buffer pH 6.0)431 - 433 nmSpec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; buffer pH 6.0; calc. on dried substance)1100 - 1300TLC-Testpasses testLoss on drying (110 °C)≤ 5 %Suitability for microscopypasses test

Legal Information

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07,GHS08,GHS09
• Signal Word: Warning
• Hazard Statements: H302 - H319 - H351 - H411
• Precautionary Statements: P201 - P202 - P273 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3