06-570-AF555
Anti-phospho-H3 Antibody (Ser10), Alexa Fluor™ 555 Conjugate
from rabbit, ALEXA FLUOR™ 555
Synonym(s):
H3S10P, Histone H3 (phospho Ser10), H3 histone, family 3A, H3.3A, H3 histone, family 3B, H3.3B
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
conjugate
ALEXA FLUOR™ 555
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer10)
Gene Information
human ... HIST1H3F(8968)
General description
Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
Immunogen
Linear peptide corresponding to phospho-Histone H3 (Ser10).
Application
Anti-phospho-H3 (Ser10), Alexa Fluor™ 555 Conjugate is a purified rabbit polyclonal Antibody that detects Histone H3 phosphorylated at serine 10. This Ab is labeled with Alexa Fluor 555 and validated for use in IC. The unconjugated version of this antibody is published & proven in ICC, IP & WB.
Immunoprecipitation Analysis: 10 µg from a representative lot of the non-conjugated form of this antibody (Cat. # 06-570) immunoprecipitated phospho-Histone H3 (Ser10) from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate.
Beadlyte Assay Analysis: A representative lot of the non-conjugated form of this antibody (Cat. # 06-570) recognized Histone H3 phosphorylated on Ser10 by Luminex assay.
Western Blot Analysis: 0.5-1 µg/mL from a representative lot of the non-conjugated form of this antibody (Cat. # 06-570) detected phospho-Histone H3 (Ser10) on 10 µg of Colcemid-treated HeLa acid extract, but not on untreated HeLa acid extract.
Beadlyte Assay Analysis: A representative lot of the non-conjugated form of this antibody (Cat. # 06-570) recognized Histone H3 phosphorylated on Ser10 by Luminex assay.
Western Blot Analysis: 0.5-1 µg/mL from a representative lot of the non-conjugated form of this antibody (Cat. # 06-570) detected phospho-Histone H3 (Ser10) on 10 µg of Colcemid-treated HeLa acid extract, but not on untreated HeLa acid extract.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Quality
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H3 (Ser10) in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H3 (Ser10) in HeLa cells.
Target description
~17 kDa observed
Physical form
Protein A purified
Purified rabbit polyclonal in buffer containing PBS with 15 mg/ml BSA and 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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