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V-014

Supelco

Thiamine HCl (Vitamin B1) solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

Synonym(s):

Thiamine hydrochloride, Vitamin B1, Aneurine hydrochloride, Vitamin B1 hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C12H17ClN4OS · HCl
CAS Number:
67-03-8
Molecular Weight:
337.27
Beilstein:
3851771
EC Number:
200-659-6
MDL number:
MFCD00012780
UNSPSC Code:
41116107
PubChem Substance ID:
329828957
NACRES:
NA.24

grade

certified reference material

Quality Level

300

form

liquid

feature

Snap-N-Spike®/Snap-N-Shoot®

packaging

ampule of 1 mL

manufacturer/tradename

Cerilliant®

concentration

1.0 mg/mL in methanol (as free base)

technique(s)

gas chromatography (GC): suitable
liquid chromatography (LC): suitable

mp

250 °C (dec.) (lit.)

application(s)

clinical testing
clinical testing

format

single component solution

storage temp.

−20°C

SMILES string

CC1=NC(N)=C(C[N+]2=CSC(CCO)=C2C)C=N1.Cl.[Cl-]

InChI

1S/C12H17N4OS.2ClH/c1-8-11(3-4-17)18-7-16(8)6-10-5-14-9(2)15-12(10)13;;/h5,7,17H,3-4,6H2,1-2H3,(H2,13,14,15);2*1H/q+1;;/p-1

InChI key

DPJRMOMPQZCRJU-UHFFFAOYSA-M

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General description

Thiamine is a water-soluble vitamin of the B complex whose phosphate derivatives are involved in many cellular processes required for overall human health. Thiamine deficiency, often the result of impaired nutritional status associated with chronic diseases from alcoholism to HIV-AIDS, is monitored in patient whole blood samples by HPLC.

Application

This certified solution standard is suitable for use as a starting material in preparation of linearity standards, calibrators, and controls in a variety of testing applications from diagnostic testing of thiamine deficiency by HPLC to quality control testing of thiamine-based dietary supplements, fortified foods or pharmaceutical preparations.

Legal Information

CERILLIANT is a registered trademark of Merck KGaA, Darmstadt, Germany
Snap-N-Shoot is a registered trademark of Cerilliant Corporation
Snap-N-Spike is a registered trademark of Merck KGaA, Darmstadt, Germany

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Pricing

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V-017

Nicotinic acid (Vitamin B3) solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

V-019

Cyanocobalamin (Vitamin B12) solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

V-052

Thiamine-13C3 (Vitamin B1-13C3) hydrochloride solution, 100 μg/mL in methanol (as free base), certified reference material, ampule of 1 mL, Cerilliant®

V-055

(-)-Riboflavin-13C4,15N2 (Vitamin B2-13C4,15N2) solution, 100 μg/mL (1% ammonium acetate in 50:50 methanol:water), certified reference material, ampule of 1 mL, Cerilliant®

V-067

(−)-Riboflavin, 100 μg/mL (1% ammonium acetate in 50:50 methanol:water), certified reference material, ampule of 1 mL, Cerilliant®

V-063

Pantothenic acid-13C3,15N hemicalcium salt solution, 100 μg/mL (90:10 Methanol:Water, (as free acid)), certified reference material, ampule of 1 mL, Cerilliant®

V-056

Riboflavin 5′-phosphate sodium salt solution, 100 μg/mL (50:50 Methanol:1% Ammonium Acetate in Water, (as free phosphate)), certified reference material, ampule of 1 mL

V-069

Pyridoxal 5′-phosphate solution, 500 μg/mL (80:20 Water:Acetonitrile), certified reference material, ampule of 1 mL, Cerilliant®

V-080

Pyridoxine-13C3 (Vitamin B6-13C3) hydrochloride solution, 100 μg/mL in methanol, certified reference material, ampule of 1 mL, Cerilliant®

F-087

Folic acid solution, 1 mg/mL in methanol with 0.1N NaOH, certified reference material, ampule of 1 mL, Cerilliant®

F-088

Folic acid-13C5,15N solution, 100 μg/mL (Methanol with 0.1 N NaOH), certified reference material, ampule of 1 mL, Cerilliant®

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

49.5 °F - closed cup

Flash Point(C)

9.7 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

HPLC Analysis of Water-Soluble B-Vitamins on Ascentis® Express C18

Pyridoxine hydrochloride, ≥98% (HPLC); Nicotinamide, ≥98.5% (HPLC); Thiamine hydrochloride, reagent grade, ≥99% (HPLC); Biotin, ≥99.0% (T); Folic acid, analytical standard; Cyanocobalamin (B12), analytical standard; Riboflavin (B2), analytical standard

Protocols

HPLC Analysis of Vitamins B1 B6 B12

HPLC Analysis of Vitamins B1, B6 and B12 using Ascentis® Express RP-Amide

LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5

Separation of Pyridoxine Hydrochloride (B6), analytical standard; Thiamine Hydrochloride (B1), analytical standard

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