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Key Documents

L032100

Sigma-Aldrich

DCI Activator

0.5 M

Synonym(s):

DCI activator solution

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About This Item

UNSPSC Code:
41116105
NACRES:
NA.21

type

for DNA synthesis
for DNA synthesis

product line

Proligo Reagents

storage condition

dry at room temperature

concentration

58-64 g/L (DCI content, titration)

impurities

≤30 ppm water content (Karl Fischer)
≤30 ppm water content (Karl Fischer)

color

clear
clear

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General description

Activator solution, containing powerful activators like 4,5-dicyanoimidazole(DCI), 5-(3,5-bis(trifluoromethyl)phenyl)-1H-tetrazole (Activator 42) or5-(Ethylthio)-1H-tetrazole (ETT) in acetonitrile, are mixed with solutions ofphosphoramidites during the extension step. The activator reacts with theamidite group to form a highly reactive intermediate. The intermediate thenforms an internucleotide bond with the detritylated 5′hydroxyl group of thegrowing oligonucleotide chain.

Preparation Note

Dicycanoimidazole /Acetonitrile 6/100 (w/v)

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

41.0 °F

Flash Point(C)

5 °C

Certificates of Analysis (COA)

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